W0177
New Approaches To Twinned Data Processing. M. Ruf, J.
Chambers, J. Kaercher, Bruker Advanced X-ray Solutions, Madison, WI,
U.S.A.
With the introduction of CCD detectors the recognition of
diffraction patterns from twinned crystals became much easier. Problems with
indexing such patterns are much more obvious with the visual feedback from
instant diffraction images. The arising interest in the crystallographic
community for handling such cases provided a stimulus for the development of
algorithms and software packages capable of visualizing reflections in the
undistorted reciprocal space, automatic indexing and data integration. Over the
years sophisticated tools were developed to unravel the tangled reciprocal
lattices of twins.
Until recently Bruker’s GEMINI was the state of the art
tool for automated indexing and data processing of integrated data from twinned
crystals. It used intensity data from SAINT derived by independent integrations
for each component of the twin. GEMINI then provided a rather crude correction
for interferring reflections. The methods of correcting for overlapping
reflections were not perfect, but allowed in many cases the determination of a
structure acceptable for publication. Another shortfall was that these methods
did not allow scaling and absorption correction.
We will present new methods for better treatment of twinned
data during integration, scaling and absorption correction. An enhanced version
of SAINT allows the simultanous integration of Bragg intensities from all twin
components. SAINT deconvolutes overlapping reflections estimating individual
intensities from their degree of overlap. Special HKL intensity files are
generated which need to be processed with TWINABS for scaling and absorption
correction. TWINABS generates HKL files for structure solution and subsequent
structure refinement.
Results from processing twinned data using SAINT and TWINABS
will be compared with methods previously available. A new graphical user
interface to SAINT will be presented providing extensive graphical feedback
about the integration process of twins.