W0211

Data Collection of Ultra-high Resolution Aldose Reductase Crystals at 15K. S. Ginell1, I. Hazemann2, A. Mitschler2, T. Petrova2, F. Ruiz2, M. Van Zandt3, A. Joachimiak1 and A. Podjarny2, 1Biosciences, Argonne National Laboratory, 9700 S. Cass Ave., Argonne, IL 60439 USA, 2IGBMC, 1 rue Laurent Fries, Illkirch, France, 3IDD, 23 Business Dr., Branford, CT.

Ultra-high resolution 0.9 Å diffraction data for Aldose Reductase inhibitor complexes was collected from helium cooled crystals at the Structural Biology Center, beamline ID19 at the Advanced Photon Source, Argonne National Laboratory. Aldose Reductase is an enzyme of MW=36 Kdaltons involved in diabetes complications. For one inhibitor, IDD 676, data was collected at 15 K, while for the second inhibitor, IDD 594, data was collected at 15 K and at 60K. In order to attain a temperature of 15K at the crystal, an open flow helium cryostat (Cryo Industries of America) was used. Diffraction data was collected at 19KeV using a 2_ offset on the SBC2 3x3 mosaic CCD detector.

The crystals showed an unusually low mosaic spread (0.15°). Initial treatment of the data give Rsym factors of 0.02 at low resolution and of 0.206 in the resolution range 0.93-0.90 Å. A signal/noise ratio of 15 overall and of 2.6 in the resolution range 0.93-0.90 Å is observed. The overall completeness is 95.1% (92.8% in the resolution range 0.93-0.90 Å). Refinement is currently in progress, and the results will be discussed.

This work was supported by the U.S. Department of Energy, Office of Biological and Environmental Research under contract No. W-31-109 ENG-38, the Centre National de la Recherche Scientifique (CNRS), by collaborative projects CNRS-CERC and ECOS-SUD, by the Institut National de la Santé et de la Recherche Médicale and the Hôpital Universitaire de Strasbourg (H.U.S), and by the Institute for Diabetes Discovery, Inc. through a contract with the CNRS.

The submitted manuscript has been created by the University of Chicago as Operator of Argonne National Laboratory (“Argonne”) under Contract No. W-31-109-ENG-38 with the U.S. Department of Energy. The U.S. Government retains for itself, and others acting on its behalf, a paid-up, nonexclusive, irrevocable worldwide license in said article to reproduce, prepare derivative works, distribute copies to the public, and perform publicly and display publicly, by or on behalf of the Government.