W0325

Structures of proteins determined at atomic resolution provide a highly detailed view of stereochemistry (especially deviations from ideal), multiple conformations, protonation states, interaction with solvent, metal ions and other ligands. Such data is critical for understanding of structure, interactions and aid drug design. However, the experiments involving data collection at atomic resolution are highly demanding. This is because there are numerous and rigorous requirements on crystal samples, data collection hardware and processing software. Crystals must be high quality, reproducible, large and radiation resistant. Among proteins that were crystallized, only a small fraction produces crystal forms that diffract to 1 Å or higher resolution. For medium and large proteins, atomic resolution data can only be obtained with synchrotron radiation. Third generation synchrotron undulator beamlines such as the Structural Biology Center, beamline ID19 at the Advanced Photon Source, Argonne National Laboratory provide new opportunities to make such highly challenging experiments more accessible to general users. Details of beamline hardware, software and strategies for atomic data collection will be discussed.

The submitted manuscript has been created by the University of Chicago as Operator of Argonne National Laboratory (“Argonne”) under Contract No. W-31-109-ENG-38 with the U.S. Department of Energy. The U.S. Government retains for itself, and others acting on its behalf, a paid-up, nonexclusive, irrevocable worldwide license in said article to reproduce, prepare derivative works, distribute copies to the public, and perform publicly and display publicly, by or on behalf of the Government.