W0454

Recent Results From the High-throughput Pipeline of the Joint Center for Structural Genomics. Qingping Xu¹, Henry van den Bedem¹, Linda Brinen¹, Ashley Deacon¹, Adam Godzik², Slawomir Grzechnik², Carina Grittini³, Scott Lesley⁴, Mitchell D. Miller¹ and Guenter Wolf¹, ¹JCSG, Stanford Synchrotron Radiation Laboratory, Stanford Univ., Menlo Park, CA, ²JCSG, San Diego Supercomputer Center, Univ. of California San Diego, La Jolla, CA, ³JCSG, The Scripps Research Institute, La Jolla, CA., ⁴JCSG, Genomics Inst. of the Novartis Research Foundation, San Diego, CA.

Structural genomics aims to significantly improve the efficiency of the protein structure determination process. One of the goals is to produce a complete catalogue of protein folding space. The Joint Center for Structural Genomics (JCSG) has developed a highly automated pipeline, which consists of target selection, cloning, purification and crystallization, crystal screening, data collection and structure determination. The JCSG pipeline has been tested on the Thermotoga maritima genome. Many novel protein fold features have already been identified. Some of latest structural results from the JCSG pipeline will be highlighted.

At SSRL we have developed a system to carry out the crystallographic analysis part of the JCSG pipeline. For many of the structures solved so far this system can be used with minimal human intervention. However, in some cases more dedicated human input has been necessary.

The JCSG pipeline is now being extended to tackle other genomes and specific classes of proteins that were not amenable to the initial pipeline.

The JCSG is funded by the Protein Structure Initiative of the National Institutes of Health, National Institute of General Medical Sciences. SSRL operations is funded by DOE BES, and the SSRL Structural Molecular Biology program by DOE BER, NIH NCRR BTP and NIH NIGMS.