Postdoctoral Research at the University of Texas, Austin
A postdoctoral position is available immediately to work on a project identifying inhibitors to the influenza protein NS1. The protein has two domains both of which are potential drug targets. We have X-ray structures of both domains as apo protein.
An ideal candidate would have experience in protein crystallography, but also in basic protein biochemistry.
The researcher would be expected to carry out X-ray analysis of complexes of the NS1 proteins with inhibitors. In addition the researcher should be able to participate in biochemical assays to screen libraries for inhibitor platforms; to date we have used a fluoresce polarization assay, but we are interested in developing secondary assays centered on ITC or DSF.
The overall project is aimed at development of antiviral leads. Our group is collaborating with a team of virologists who do cell based screening of inhibitors, another team involved with the chemical synthesis of derivatives designed from screens and structural analysis.
Please send a resume and list of at least 3 referees to:
Dr. Jon Robertus
Institute of Cellular and Molecular Biology
Department of Chemistry and Biochemistry
jrobertus@mail.utexas.edu
For email responses please list postdoctoral applicant in the subject line.
POSTDOCTORAL POSITIONS
cold Spring Laboratory
Applications are invited for postdoctoral positions at Cold Spring Harbor Laboratory (CSHL) from highly enthusiastic individuals with strong interest in utilizing structural, electrophysiological, and biochemical approaches to study fundamental problems in ion and substrate transports across the membrane. Our goal is to understand molecular basis for synaptic transmission and neurological disorders and diseases. We use x-ray crystallography to obtain atomic structures of target membrane/glyco proteins and test structure-based functional hypotheses by various techniques including electrophysiology, analytical centrifugation, and fluorescence assay. We are equipped with a state-of-the-art facility to conduct membrane/glyco protein crystallography and functional studies including a high-throughput protein screening system, an automated crystallization screening device, a recently updated home x-ray source, electrophysiology rigs, and other setups such as isothermal titration colorimetry device. CSHL is also located within 30 min driving distance from the National Synchrotron Light Source at Brookhaven National Laboratory. Most importantly, a highly collaborative environment at CSHL provides a unique opportunity for postdocs to interact with scientists from various fields including cancer biology, neuroscience, and plant biology. Candidates must hold (or soon expect to hold) PhD in biochemistry, molecular biology, biophysics or related fields. Strong background in molecular biology, biochemistry, large-scale protein expression in eukaryotic and prokaryotic cells, and purification of membrane/glyco proteins is desired. Experience in x-ray crystallography or electrophysiology is desirable but not necessary. For more information, please check the laboratory website (http://www.cshl.edu/public/SCIENCE/furukawa.html) and contact me via email. Please send CV, a summary of research experience and interest, and three references to Hiro Furukawa by email at furukawa@cshl.edu
POSTDOCTORAL POSITIONS
DEPT. OF BIOCHEMISTRY, VANDERBILT UNIVERSITY
Two postdoctoral positions funded by US NIH grants are available immediately to work on structural studies of native and chemically modified nucleic acids, protein-nucleic acid interactions or the KaiABC cyanobacterial circadian clock. An overview of current projects in our laboratory can be found at: http://structbio.vanderbilt.edu/~eglim/
For recent publications in the areas of DNA, RNA and protein-nucleic acid interactions, please see: J. Am. Chem. Soc. 2009, 131, p12548; J. Biol. Chem. 2009, 284, p22467; Chem. Comm., 2009, p2017; RNA 2008, 14, p2125; Cell Cycle 2008, 7, p2562; J. Biol. Chem. 2007, 282, p36421; Nucleic Acids Res. 2007, 35, p6424; Annu. Rev. Biophys. Biomol. Struct. 2007, 36, p281; Acc. Chem. Res. 2007, 40, p197; J. Am. Chem. Soc. 2006, 128, p10847. For recent publications focusing on the structure/function of the KaiABC clock, please see: Science 2008, 322, p697; EMBO J. 2008, 27, p1767; PLoS Biol. 2007, 5, e93; EMBO J. 2006, 25, p2017; Proc. Natl. Acad. Sci. USA 2004, 101, p13933; Nature Struct. Molec. Biol. 2004, 11, p584; Mol. Cell 2004, 15, p375.
All crystallographic data collections are being conducted at the Advanced Photon Source, Argonne National Laboratory, to which we have extensive access via the Life Sciences CAT at sector 21. Candidates must have a recent Ph.D. and a strong background in chemistry or biochemistry as well as a basic knowledge of X-ray crystallography.
Please e-mail your curriculum vitae and the names of three references to: Prof. Martin Egli, Department of Biochemistry, Vanderbilt University, School of Medicine, Nashville, TN 37232, USA. E-MAIL: martin.egli@vanderbilt.edu
Kelly Scientific / Research Fellow Opportunity
Kelly Scientific has an immediate direct hire opportunity for a research fellow to join a laboratory in Ann Arbor, MI. The research projects involve the study of proteins using x-ray crystallography as well as biochemical, biophysics, and chemical genomics techniques. The research is focused on a number of synaptic adhesion molecules known to play a role in several neuro-psychiatric disorders and on a transcription factor that mediates neuro-adaptive and behavioral changes following chronic drug abuse.
Experience techniques needed include, but not limited to: macromolecular crystallization, purifying proteins, protein gel electrophoresis, expressing recombinant proteins, DNA gel electrophoresis, standard cloning techniques, and growing bacterial cultures and insect cell cultures.
Qualifications include a PhD in life sciences earned within the past 3 years. Relevant experience in the study of macromolecular crystallography is highly desirable. Good written and oral communication skills are essential. Interested candidates should e-mail their CV and 3 references to Mamie_Denton@kellyscientific.com.
Mamie Denton
Senior Scientific Recruiter
Kelly Scientific Resources
248-848-9360
248-848-9315 (fax)
Mamie_Denton@KellyScientific.com
www.kellyscientific.com
X-RAY FACILITY MANAGER POSITION: We are seeking an experienced person to manage our macromolecular X-ray crystallography facility and report directly to the Director (Joanne I. Yeh, Ph.D.) and co-Director (William Furey, Ph.D.). The successful candidate will train personnel in the use of X-ray crystallographic equipment, software, and robotics providing scientific and technical guidance as needed, as well as coordinate service issues with the equipment vendors. The facility manager will also coordinate scheduling and allocation of general users time and maintain usage logs, with the guidance of the Director & co-Director. The facility houses two high intensity FR-E generators with four detectors, including two Saturn 944 CCDs, a RAXIS IV++ image plate detector, and a RAXIS HTC image plate detector, along with a crystallization robot and imaging systems. An ACTOR robot is present on one system for automatic mounting and screening of crystals. All systems are equipped with VariMax optics to provide high intensity and highly focused X-ray beams, and utilize X-stream 2000 cryogenic systems. The successful candidate will also have opportunities to work in a multi-disciplinary team on structural biology projects related to the Pittsburgh Center for HIV-Protein Interactions. Experience with handling, cryo-cooling, and manipulation of macromolecular crystals and in structure determination and refinement is required. Prior X-ray facilities management or equipment maintenance experience is a plus. Applicants should have a PhD or equivalent experience and salary is to be commensurate with experience.
Interested applicants should send their CV, the names of 3 references, and have letters sent to either:
Dr. Joanne I. Yeh
Department of Structural Biology
University of Pittsburgh School of Medicine
3501 Fifth Avenue
1036 BST3
Pittsburgh, PA 15260
Office: 412-648-9027
E-mail: jiyeh@pitt.edu
or
Dr. William Furey
Dept. of Pharmacology & Chemical Biology
University of Pittsburgh School of Medicine
Lothrop Street
1340W BSTWR
Pittsburgh, PA 15261
Tel: 412-683-9718
E-mail: fureyw@pitt.edu
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